Setup and Bright Field Alignment

7- Focus specimen
Use the coarse focus knob (M) the bring the specimen into rough focus. Once you've got the best possible focus with the coarse focus knob (M) use the fine focus knob (N). Do not force the coarse focus knob - you don't want to ram it through the slide and damage the objective and slide. This can be a problem with thick preparations such as whole mounts and wet mounts.